Abstract
Betahistine is a novel drug used to treat vertigo. The research focuses on developing and optimizing a simple, environmentally sustainable spectrophotometric approach for the analysis of BH in tablet formulations. The present investigation is based on the reaction between BH and potassium iodide and potassium iodate, monitored by Ultraviolet-Visible spectrophotometry at 352 nm. The most influential variables were optimized using response surface methodology via Box–Behnken design, resulting in reduced chemical, energy, and analysis time, and ultimately the analysis cost. Environmental sustainability was assessed using Analytical Eco-Scale (92), Analytical Greenness (0.72), Blue Applicability Grade Index (72.5) and Environmental and Practical Performance Index (89.1) metrics, which confirmed outstanding greenness compared with other reported methods. The method was validated as per International Conference on Harmonization (ICH) guidelines. The linearity, LOD and LOQ values were 2.5–12.5, 1.25 and 3.45 μ g/mL, respectively. The accuracy and precision were also excellent within the range. Moreover, the simplicity, reproducibility, and low-cost instrumental analysis of the drug enhance its wide applicability and provide a reliable solution for quality control in BH commercial formulations, chemical and pharmaceutical research laboratories, and academicians.
Recommended Citation
Haque, Shaikh Manirul
(2026),
Box-Behnken Design-Based Response Surface Modelling for the Optimized Greener Spectrophotometric Methodology to Determine Betahistine in Pharmaceutical Formulations,
Yanbu Journal of Engineering and Science: Vol. 23:
Iss.
1, 142-153.
DOI: https://doi.org/10.53370/3122-3303.1215
Available at:
https://yjes.researchcommons.org/yjes/vol23/iss1/9
Supplementary File
